СПОСОБ ПОЛУЧЕНИЯ КОНЦЕНТРАТА КУЛЬТУРЫ КЛЕТОК БРУЦЕЛЛ ИЗ ШТАММА Brucella abortus 19 ДЛЯ ПРИГОТОВЛЕНИЯ БРУЦЕЛЛЕЗНЫХ АНТИГЕНОВ, БРУЦЕЛЛЕЗНЫЕ АНТИГЕНЫ (ТРИ ВАРИАНТА), СПОСОБ ИЗГОТОВЛЕНИЯ БРУЦЕЛЛЕЗНОЙ ДИАГНОСТИЧЕСКОЙ СЫВОРОТКИ И ТЕСТ-СИСТЕМЫ ДЛЯ ДИАГНОСТИКИ БРУЦЕЛЛЕЗА ЖИВОТНЫХ (ТРИ ВАРИАНТА)
Trojnin Anatolij Serafimovich,Тройнин Анатолий Серафимович,Elnikov Vasilij Viktorovich,Ельников Василий Викторович,Kryukova Elena Nikolaevna,Крюкова Елена Николаевна,Surnev Dmitrij Sergeevich,Сурнев Д
申请号：
RU2014139329/10
公开号：
RU0002593712C2
申请日:
2014.09.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
FIELD: biology.SUBSTANCE: presented group of inventions relates to a method for preparing concentrated Brucella cell culture from Brucella abortus 19 strain to prepare brucellous antigens, a method for preparing concentrated Brucella cell culture from Brucella abortus 19 strain to prepare brucellosis diagnostic serum, a method of producing a single brucellar antigen in agglutination test (AT), complement-fixation test (CFT) and prolonged complement fixation test (PCFT), a method of producing brucellar antigen for rose bengal test (RBT), a method of producing brucellar antigen for milk ring test (MRT), a method of producing brucellosis diagnostic serum, a test system for diagnosing brucellosis in animals in AT, CFT and PCFT, a test system for diagnosing brucellosis in animals in RBT and a test system for diagnosing brucellosis in animals in MRT. Described method for preparing concentrated Brucella cell culture from Brucella abortus 19 strain to prepare brucellous antigens used in diagnostic test systems involves obtaining a bacterial cell inoculum and cultivation thereof in deep conditions on a liquid nutrient medium with controlled level of concentration of dissolved oxygen in the culture medium during the whole process of cultivation, inactivation of grown bacterial cells by heating with subsequent concentration. Herewith the process of bacterial cells cultivation is carried out at the temperature of 36-38 °C, in the process of cultivation at the stage of accumulation of bacterial cells from the concentration of 1.5-2.5 billion m.c./cm3 to the concentration of 10 billion m.c./cm3 the concentration of dissolved oxygen in the culture medium is maintained within the range of 20-25 mg/l, and over 10 billion m.c./cm3 and till the bacterial cells reach the stationary growth phase the concentration of dissolved oxygen in the culture medium is provided within the range of 40-45 mg/l, and the concentration of grown bacterial cells is carried out by deposition with a floccul
