A microkeratome cutting head includes a head structure, an applanation plate, and a stromal plate. The head structure may include a blade cavity for receiving a blade and a blade opening through which the blade extends. The blade is configured for creating a corneal flap at a corneal flap thickness. The applanation plate is disposed at an elevation above the stromal plate plane. The elevation difference is approximately equal to the corneal flap thickness. An artificial anterior chamber (AAC) system is used to hold the donor eye for interfacing with the microkeratome. The AAC system includes an optional pressure valve and pressure gauge in a closed loop system for the user or surgeon to control line pressure applied to the donor eye during the procedure.