The present invention provides a nucleotide sequence and an amino sequence of CDR3 region of a T cell receptor (TCR) gene, the T cell being a WT1specific cell harmful T cell (CTL) against WT1 protein. The present invention also provides a method for detecting or treating cancer by using the nucleotide sequence and amino sequence described above, a chip, a primer set, a kit and a device etc. containing the nucleotide sequence and amino sequence described above.本發明係提供抗WT1蛋白質之WT1特異性細胞傷害性T細胞(CTL)之T細胞受體(TCR)基因的CDR3區域的核苷酸序列及胺基酸序列。本發明亦提供使用該等核苷酸序列、胺基酸序列之癌檢查或癌治療之方法,含有該等核苷酸序列、胺基酸序列之用於癌檢查之晶片、引子組、套組、裝置等。