The present invention relates to a method of culturing primitive-like macrophages from stem cells, a kit when used in the method thereof and uses of the primitive like macrophage for in-vitro disease models and for screening compounds for therapy. One embodied culture method comprises contacting and incubating embryonic stem cells or induced pluripotent stem cells with a serum-free culture media comprising a GSK3 inhibitor to differentiate stem cells into cells of the mesoderm lineage, followed by incubation with a culture media comprising Dickkopf-related protein 1 (DKK1) to differentiate the mesoderm into cells of hematopoietic lineage, maturing hematopoietic cells and incubating these cells with a culture media comprising M-CSF to drive differentiation into primitive-like macrophages. Another embodiment comprises incubating the stem cells with serum-free culture media comprising FGF2 and BMP4 to induce differentiation into cells of the mesoderm lineage, followed by incubating the cells with a culture media comprising FGF2, BMP4, Activin A and VEGF to differentiate the cells of the mesoderm lineage into cells of the hematopoietic cell lineage, maturing the cells of the hematopoietic cell lineage and lastly, incubating the matured hematopoietic cells with culture media comprising M-CSF to drive the differentiation of hematopoietic cells into primitive-like macrophages.La présente invention concerne un procédé de culture de macrophages de type primitif à partir de cellules souches, un kit lorsqu'il est utilisé dans le procédé correspondant et des utilisations du macrophage de type primitif dans des modèles de maladies in vitro et pour le criblage de composés thérapeutiques. Selon un mode de réalisation, l'invention concerne un procédé de culture consistant à mettre en contact et à incuber des cellules souches embryonnaires ou des cellules souches pluripotentes induites dans des milieux de culture exempts de sérum comprenant un inhibiteur de GSK3 pour différencier le
