MARTEYN, Benoit,COIC, Yves-Marie,BALEUX, Françoise,SANSONETTI, Philippe
申请号：
EPEP2012/067587
公开号：
WO2013/034749A1
申请日:
2012.09.07
申请国别(地区):
WO
年份:
2013
代理人:
摘要：
The invention relates to polypeptides, defined through a consensus sequence, having a length from 10 to 80 amino-acid residues, and whose polypeptidic sequence comprises or consists of the consensus sequence P1 (Xa)P3(Xb)P5(Xc)P6(Xd)P7, presenting specific patterns. The polypeptides of the invention target glycosylated Muc2 proteins. The invention also relates to methods of synthesis of such polypeptides, to their nucleic acids and uses thereof. The polypeptidic sequence of the polypeptides of the invention can be part of the N-terminal sequence of a mucus-binding (MUB) domain, especially a mucus-binding (MUB) domain of several species. The invention also relates to chimeric molecule(s) comprising such polypeptides, which are labelled, and vectors, especially plasmids and population of cells or composition comprising polypeptides of the invention. Synthesis methods encompass biotechnological or chemical production. Polypeptides of the invention can be used in staining experiments, as a probe or marker for staining Muc2 protein(s) contained in mucus layer(s), to detect in vitro mucus production or mucus composition in human colon or monitoring any one of the following disease conditions: neoplasic disease(s), including mucinous carcinoma(s), gastric cancer(s) or colorectal cancer(s), especially colon cancer(s), cystic fibrosis, intestine inflammatory disease(s) such as inflammatory bowel disease (IBD) and ulcerative colitis. The invention also relates to a method for manufacturing a medicament. In a particular embodiment, use of polypeptides of the invention can be made for marking neutrophiles.Linvention concerne des polypeptides définis par une séquence consensus ayant une longueur allant de 10 à 80 résidus dacides aminés et dont la séquence polypeptidique comprend ou consiste en la séquence consensus P1 (Xa)P3(Xb)P5(Xc)P6(Xd)P7, présentant des motifs spécifiques. Les polypeptides de linvention ciblent des protéines Muc2 glycosylées. Linvention concerne également d
