JHON, YOUNG MIN,전영민,JANG, CHI WOONG,장지웅,CHUN, MYUNG SUK,전명석,LEE, SEOK,KIM, JAE HUN,김재헌,KIM, CHUL KI,김철기,WOO, DEOK HA,우덕하,LEE, TAIK JIN,이택진,BYUN, YOUNG TAE,변영태,KIM, SUN HO,김선호
申请号：
KR1020130000695
公开号：
KR1013693920000B1
申请日:
2013.01.03
申请国别(地区):
KR
年份:
2014
代理人:
摘要：
The present invention relates to a method for analyzing coupling efficiency of adhesive nano particles, comprising the steps of (a) injecting a solution including nano particles into a first chamber slide (b) vaporizing only the solution from the first chamber slide into which the solution including nano particles is injected and irradiating light from a light source to measure a saturation temperature by a thermal observation acquisition device (c) injecting cells into a second chamber slide (d) injecting the solution including nano particles into the second chamber slide in which the cells are cultured (e) removing the nano particles which are not coupled to the cells from the second chamber slide into which the cells and nano particles are injected (f) vaporizing only the solution from the second chamber slide from which the nano particles are removed and irradiating light from the light source to measure the saturation temperature by a thermal observation acquisition device and (g) comparing the number per area of the nano particles injected into the first chamber slide and the number per area of the nano particles injected into the second chamber slide at the same saturation temperature between the step (b) and the step (f).COPYRIGHT KIPO 2014[Reference numerals] (a,b,c,d) Measure a saturation temperature according to concentration (AA) GNP solution (BB) High concentration (CC) Low concentration (DD,HH,OO,TT) Temperature (EE,II,PP,UU) Saturation temperature (FF,JJ,QQ,VV) Time (GG) Medium solution (KK) Cell medium solution (LL) Combined GNP solution (MM,RR) GNP high concentration (NN,SS) GNP low concentration본 발명은 결합형 나노입자의 결합효율 분석방법에 관한 것으로, (a) 나노입자가 포함된 용액을 제1 챔버슬라이드에 주입하는 단계, (b) 상기 나노입자가 포함된 용액이 주입된 제1 챔버슬라이드에서 용액만 증발시킨 후, 광원으로부터 빛을 조사하면서 열상획득장치로 포화온도를 측정하는 단계, (c) 세포를 제2 챔버슬라이드에 주입하는 단계, (d) 상기 세포가 배양된 제2 챔버슬라이드에 나노입자가 포함된 용액을 주입하는 단계, (e) 상기 세포와 나노입자가 주입된 제2 챔버슬라이드로부터 세포에 결합되지 않은 나노입자를 제거하는 단계, (f) 상기 나노입자가 제거된 제2 챔버슬라이드로부터 용액만 증발시킨 후, 광원으로부터 빛을 조사하면서 열상획
