PROBLEM TO BE SOLVED: To sterilize a cell culture device efficiently.SOLUTION: Hydrogen peroxide solution and compressed air are sprayed into a vaporization chamber (16c), sprayed hydrogen peroxide solution is heated to boiling point or higher and completely vaporized by heating means arranged in a vaporization chamber (16c). Then, after at least an operation section (3) which performs a culture operation of cells, culture sections (4a,4b) which culture the cells, and a carry in/out storage section (2) for carrying in/out materials and tools are sterilized by spraying the solution, cell culture is started.COPYRIGHT: (C)2014,JPO&INPIT【課題】細胞培養装置を効率的に無菌化する。【解決手段】過酸化水素水と圧縮エアとを気化室(16c)内にスプレーし、スプレーされた過酸化水素水を気化室(16c)に配置された加熱手段によって沸点以上に加熱して完全に気化させた後、これを細胞の培養操作を行う操作部(3)、細胞の培養を行う培養部(4a,4b)、及び材料及び器具を出し入れするための入出庫部(2)の少なくとも三者に噴霧し殺菌した後に細胞培養を開始する。【選択図】図1
