Provided is a holographic three-dimensional multi-spot light stimulation device capable of rapidly sensing the three-dimensional positions of a plurality of target objects, and imparting light stimulation simultaneously to the plurality of target objects, which are positioned three-dimensionally, on the basis of three-dimensional fluorescence distribution information that has been obtained. The light stimulation device is provided with: a three-dimensional imaging holographic optical system A which employs fluorescent exciting light to acquire three-dimensional fluorescence distribution information resulting from fluorescent signal light from a plurality of stimulation target objects； and a three-dimensional light stimulation holographic optical system B which employs a light stimulation hologram generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, to impart stimulation simultaneously to the plurality of stimulation target objects. Furthermore, the three-dimensional light stimulation holographic optical system B is provided with a spatial light phase modulating element 22 and a control unit 25, wherein the control unit 25 generates the light stimulation hologram by controlling the spatial light phase modulating element 22 on the basis of the three-dimensional fluorescence distribution information.L'invention concerne un dispositif de stimulation lumineuse multipoints tridimensionnelle holographique permettant de détecter rapidement les positions tridimensionnelles d'une pluralité d'objets cibles et d'appliquer une stimulation lumineuse simultanément sur la pluralité d'objets cibles, positionnés en trois dimensions, en fonction d'informations de répartition de fluorescence tridimensionnelles qui ont été obtenues. Le dispositif de stimulation lumineuse selon l'invention comprend : un système optique holographique d'imagerie tridimensionnelle A qui utilise une lumière d'excitation fl
