Systems and methods are provided for cleaning a cloth or fabric that has been used for growing plants. The cloth or fabric may be separated from or mounted with respect to a tray or support during the cleaning operation. At least two opposed, rotating brushes engage the top and bottom surfaces of the cloth or fabric to abrade and clean those surfaces. In addition, spray units, e.g., high pressure fluid nozzles, may direct cleaning fluid against one or both surfaces of the cloth or fabric. Filtration and sterilization systems may also be associated with the cleaning operation.