PROBLEM TO BE SOLVED: To provide a culture method capable of massively propagating hair follicle stem cells.SOLUTION: There are provided a method of culturing hair follicle stem cells which includes a first process of culturing hair follicle stem cells in a medium containing a topoisomerase II inhibitor and a second process of culturing hair follicle stem cells in a medium containing an ALK5 inhibitor and a TGF-β inhibitor a method for culturing skin stem cells which includes a process in which the medium further includes 3,3, 5-triiodo-L-thyronine or its salt and skin stem cells are isolated after cultivation a composition for culturing hair follicle stem cells which includes an ALK5 inhibitor and a Rock inhibitor and a hair growing agent which contains an ALK5 inhibitor and a Rock inhibitor as active constituents.COPYRIGHT: (C)2013,JPO&INPIT【課題】毛包幹細胞を大量に増殖させることができる培養方法を提供する。【解決手段】毛包幹細胞を、トポイソメラーゼII阻害剤を含む培地において培養する第1工程と、毛包幹細胞を、ALK5阻害剤とTGF-β阻害剤とを含む培地において培養する第2工程と、を含む、毛包幹細胞の培養方法。培地がさらに3,3,5-トリヨード-L-チロニン又はその塩を含み、培養後、皮膚幹細胞を単離する工程を含む、皮膚幹細胞の培養方法。更にALK5阻害剤とRock阻害剤とを含む、毛包幹細胞培養用組成物。及びALK5阻害剤とRock阻害剤とを有効成分として含有する発毛剤にも関する。【選択図】なし
