The present invention provides isolated monoclonal antibodies that specifically bind LAG-3, and have optimized functional properties compared to previously described anti-LAG-3 antibodies, such as antibody 25F7 (US 2011/0150892 A1). These properties include reduced deamidation sites, while still retaining high affinity binding to human LAG-3, and physical (i.e., thermal and chemical) stability. Nucleic acid molecules encoding the antibodies of the invention, expression vectors, host cells and methods for expressing the antibodies of the invention are also provided, as well as immunoconjugates, bispecific molecules and pharmaceutical compositions comprising the antibodies. The present invention also provides methods for detecting LAG-3, as well as methods for treating stimulating immune responses using an anti-LAG-3 antibody of the invention. Combination therapy, in which the antibodies are co-administered with at least one additional immunostimulatory antibody, is also provided.本發明提供經分離單株抗體，其特異性結合LAG-3且與先前所闡述抗LAG-3抗體(例如抗體25F7，US 2011/0150892 A1)相比具有最佳化功能性質。該等性質包含在保留對人類LAG-3之高親和力結合的同時減少之去醯胺化位點及物理(亦即熱及化學)穩定性。亦提供編碼本發明該等抗體之核酸分子、用於表現本發明該等抗體之表現載體、宿主細胞及方法，以及包括該等抗體之免疫偶聯物、雙特異性分子及醫藥組合物。本發明亦提供檢測LAG-3之方法以及使用本發明之抗LAG-3抗體刺激免疫反應之治療方法。亦提供組合療法，其中共投與該等抗體與至少一種其他免疫刺激性抗體。
