A specimen retrieval assembly including a housing containing a specimen retrieval bag and pliable spring, a distal end including a non-blunt tip, and a deployment mechanism. The specimen retrieval bag is moved from the stowed position within a hollow housing to a deployed position by sliding a rod from a retracted position partially within the hollow housing to an extended position that projects at least partially out of the hollow housing. Two portions of a flexible support ring unfold under spring bias from a folding position as the rod enters the extended position to open a mouth of the specimen retrieval bag. The distal end of the assembly has a non-blunt tip which is shaped to puncture human skin.