Maria Rosaria Romano;Paolo Costantino;Francesco Berti
发明人:
Paolo Costantino,Maria Rosaria Romano,Francesco Berti
申请号：
US13504920
公开号：
US20120282295A1
申请日:
2010.11.01
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants； diafiltration, e.g. to remove low molecular weight contaminants； anion exchange chromatography, e.g. to remove residual protein； and concentration.
