The present disclosure involves optimized methods for production of biologically active proteins termed optimally manufactured stradomers. The present disclosure further provides compositions and methods useful in the treatment of diseases and conditions including autoimmune diseases, inflammatory diseases, or infectious diseases.本發明涉及產生稱為最佳製備之斯塔都聚體(stradomer)之生物活性蛋白的最佳化方法。本發明進一步提供可用於治療疾病及病況、包括自體免疫疾病、發炎性疾病或傳染性疾病之組合物及方法。