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Genetic engineering of non-human animals for the production of chimeric antibodies
专利权人:
ABLEXIS LLC
发明人:
SHIZUYA HIROAKI,GREEN LARRY
申请号:
NZ60117111
公开号:
NZ601171A
申请日:
2011.03.31
申请国别(地区):
NZ
年份:
2014
代理人:
摘要:
Disclosed is a method of producing a mouse cell comprising a genome that comprises a functional polynucleotide sequence that encodes a chimeric immunoglobulin heavy chain that functionally replaces an endogenous immunoglobulin heavy chain locus, wherein said chimeric immunoglobulin heavy chain comprises a human variable domain and a chimeric constant region, said method comprising the steps of: designing a DNA construct in silico, wherein said construct comprises one or more unrearranged human V, D and/or J gene segments, a human CH1 gene segment, a human upper hinge region gene segment, a mouse CH2 gene segment, and a mouse CH3 gene segment; producing said one or more DNA constructs; and introducing the one or more constructs into the genome of a cell. Further disclosed is a mouse cell as per the method as disclosed above or a mouse contain said cell.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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