The present invention relates to the technical field of health foods, in particular to an AGEs inhibitory composition and its use, preparation method and formulation. The AGEs inhibitory composition, in terms of parts by weight, consists of the following raw materials: bilberry extract 25 ∼ 500 parts, ginkgo biloba extract 15 ∼ 110 parts, green tea extract 25 ∼ 125 parts, rosemary extract 0.3 ∼ 20 parts, vitamin B1 0.1 ∼ 10 parts, vitamin B2 0.1 ∼ 10 parts, vitamin B6 0.1 ∼ 10 parts. The formula of the AGEs inhibitory composition of the present invention is reasonable, and the combination of bilberry extract, ginkgo biloba extract, green tea extract, rosemary extract, vitamin B1, vitamin B2, and vitamin B6 can produce a significant synergistic effect in inhibiting AGEs production and degrading AGEs.