A method for disinfecting of soil or other substrates for agricultural crop,characterized in that it comprises: obtaining a soil or another substrate foragricultural crop in its field capacity; treating the soil or substrate in itsfieldcapacity from the previous stage with ozonated water, wherein the ozonatedwater is prepared in situ with an ozone-producing apparatus connected to thewater supply; allowing a period of time to pass after treatment with ozone;andinoculating the disinfected soil or agricultural substrate with at least onespeciesof beneficial microorganisms.