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METHOD FOR INCREASING THE CELL ANTIGEN ETEC CS6 PRESENTATION AND PRODUCTS OBTAINED ON ITS BASIS
专利权人:
СКАНДИНАВИАН БАЙОФАРМА ХОЛДИНГ АБ
发明人:
КАРЛИН Нильс,СВЕННЕРХОЛЬМ Анн-Мари,ТОБИАС Йошуа
申请号:
RU2014114284
公开号:
RU2014114284A
申请日:
2012.09.10
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A method of enhancing the presentation of CS6 ETEC antigen on a cell surface, comprising the step of bringing cells expressing said antigen into contact with an aqueous solution containing 0.6-2.2 percent phenol by weight, so that the presentation of said antigen is increased by at least at least 100% .2. The method of claim 1, wherein the presentation of said antigen is increased by at least 200%, preferably at least 300%. The method of claim 1, wherein the duration of the contacting step is from 1 to 72 hours. The method of claim 3, wherein the duration of the contacting step is from 1.5 to 42 hours. The method of claim 3, wherein the temperature during the contacting step is from 18 to 42 ° C. 6. The method of claim 3, wherein the phenol concentration is 0.8-2.0 percent by weight. The method of claim 5, wherein the phenol concentration is 0.8-2.0 percent by weight. The method of claim 1, wherein the phenol concentration is 0.6-2.0 percent by weight, the contact time is 6-72 hours, and the contact temperature is 18-22 ° C. The method of claim 8, wherein the phenol concentration is 0.75-0.85 percent by weight, the contact time is 40 ± 2 hours, and the contact temperature is 20 ± 1ºC. 10. The method according to any one of paragraphs. 1-7, where the temperature during the contacting stage is from 18 to 42ºC. 11. The method of claim 10, wherein the temperature during the contacting step is from 18 to 38ºC, preferably 18-22ºC or 36-38ºC. The method according to any one of paragraphs. 1-7, where the concentration of phenol is 0.8-2.0 percent by weight, preferably 1.0-2.0 percent by weight. The method according to any one of paragraphs. 1-9, where the cells include Escherichia coli cells. 14. The method according to any one of paragraphs. 1-9, where the antigen is recombinantly1. Способ повышения презентации антигена CS6 ETEC на клеточной поверхности, включающий стадию приведения клеток, экспрессирующих указанный антиген, в контакт с водным раствором, содержащим 0,6-
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