The present disclosure provides antibodies and antigen-binding fragments thereof that bind to human OX40 (ACT35, CD134, or TNFRSF4), a pharmaceutical composition comprising said antibody, and use of the antibody or the composition for treating a disease, such as cancer. In particular, the anti-OX40 antibody of the present invention does not interfere with the binding of OX40-ligand to its receptor.本公開文本提供了與人OX40(ACT35、CD134或TNFRSF4)結合的抗體及其抗原結合片段、包含所述抗體的醫藥組合物以及所述抗體或所述組合物用於治療疾病如癌症的用途。特別地,本發明的抗OX40抗體不干擾OX40配體與其受體的結合。