Biofluid collection device includes acquisition module and shell. Acquisition module includes a shell, and shell includes that first end is open with sample introduction, and second end has sample distribution opening, the channel extended between opening, and sample and sample distribution are open. Mixing chamber and the channel for keeping room to be in fluid communication pass through mixing chamber and subsequently enter the room so that sample introduces sample introduction opening and slips over. Biofluid collection device further includes closure dam first end, and the second end cap covering includes breather plug, actuating component be suitable for making containing sample keep removing sample distribution opening in room. Collection module is positioned at shell and the open end of lid closes the housing.