An object of the present invention is to provide a stemcell applicable to regenerative therapeutic method, and toprovide a technique to carry out regenerative therapy using thecell. A collected cardiac tissue fragment is enzymaticallytreated to prepare a cell suspension. Then using the cellsuspension, following steps are carried out:(1) separation ofcells by the density gradient method, (2) suspension-culture ina culture medium containing fibroblast growth factor andepidermal growth factor and (3) selection and separation ofcells forming a floating sphere to obtain pluripotent stem cells.Thus-obtained pluripotent stem cells are used to carry outregenerative therapy.
