Activated antigen-presenting cells that can induce immunocytes including disease antigen-specific CD8+ CTLs and/or ³´ T cells efficiently in vivo and/or in vitro, a medical composition comprising the activated antigen-presenting cells, a treatment and prevention method using the activated antigen-presenting cells, and an induction method of immunocytes including disease antigen-specific CTLs and/or ³´ T cells induced using the activated antigen-presenting cell, immunocytes induced by the above-noted method, a medical composition comprising the immunocytes, and a treatment and prevention method using the immunocytes are provided. By co-pulsing antigen-presenting cells with bisphosphonate in addition to the pulse with a disease antigen, the ratio of disease antigen-specific CD8+ CTLs and/or ³´ T cells and the number of the disease antigen-specific CD8+ CTLs and the ³´ T cells can be increased, compared with the case where the co-pulse with bisphosphonate is not carried out.
