FROST, GREGORY IAN,GUIBINGA, GHIABE H.,ONUFFER, JAMES JOSEPH,HAERIZADEH, FARZAD
申请号：
CA3030003
公开号：
CA3030003A1
申请日:
2017.07.08
申请国别(地区):
CA
年份:
2018
代理人:
摘要：
The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and/or NK cells. The methods can include inhibitory RNA molecule(s) and/or engineered signaling polypeptides that can include a lymphoproliferative element, and/or a chimeric antigen receptor (CAR), for example a microenvironment restricted biologic CAR (MRB-CAR). Additional elements of such engineered signaling polypeptides are provided herein, such as those that drive proliferation and regulatory elements therefor, as well as replication incompetent recombinant retroviral particles and packaging cell lines and methods of making the same. Numerous elements and methods for regulating transduced and/or genetically modified T cells and/or NK cells are provided, such as, for example, those including ribos witches, MRB-CARs, recognition domains, and/or pH-modulating agents.
