A fungus cultivation device includes at least one accommodating structure and a light-emitting diode module. The accommodating structure is disposed with a main body having an opening. The main body is internally disposed with an accommodating space for accommodating fungi strains and a light-permeable cover for covering the opening so as to seal the accommodating space. A plurality of light-emitting diodes of the light-emitting diode module irradiate the accommodating structure to supply a light source for the fungus inside the accommodating structure, thereby facilitating fungus growth.