A toxicity detection device and method for a wild plant. The device comprises an upper cover (1), a cylindrical body (14), and a motor (11). A cavity of the cylindrical body (14) is provided with a rotating blade device (13) therein. The motor (11) is connected to the rotating blade device (13) via a conductive wire. The motor (11) drives the rotating blade device (13) to rotate and shred a wild plant in the cylindrical body (14) to form a plant liquid. Multiple agent cylinders (5) are provided on a side wall of the cylindrical body (14). Each of the agent cylinders (5) is connected to a glass cylinder (9) via a communication tube (8). The cylindrical body (14) is provided with multiple holes (140) at a bottom portion thereof, each of the holes (140) corresponding to one glass cylinder (9). The plant liquid in the cylindrical body (14) passes through the holes (140) to flow into the corresponding glass cylinders (9). An electric ring (7) is provided on a side wall of the cylindrical body (14). Each communicat