A seine net calibration target for an image sensor can include a given set of seine nets connected to form a polygon and another set of seine nets. Each seine net of the other set of seine nets is coupled to a vertex of the polygon at a given end and coupled to a respective one of a plurality of boats at another end. The seine net calibration target can also include a plurality of light sources arranged along a top edge of the given set of seine nets and the other set of seine nets. The plurality of light sources emits light at a predetermined wavelength.