A method for transforming plastids in plant cells, the method comprises: 1) introducing into the nucleus of said plant cell a nucleic acid comprising a nuclear promoter linked plant operably linked to a first nucleic acid sequence comprising a cassette plastid transgene, a sequence of translocation of plastids (PTS) that is an RNA sequence capable of binding to a binding protein PTS plant, and a binding domain primer selected from that of a retrotransposon or retrovirus； 2) introducing into the nucleus of said plant cell a second nucleic acid sequence encoding a binding protein translocation sequence fused plastids a first transit peptide plastid, wherein said second nucleic acid sequence is operably linked a nuclear plant promoter； and 3) introducing into the nucleus of said plant cell a third nucleic acid sequence encoding a protein fused reverse transcriptase to a second peptide plastid transit, wherein the third nucleic acid sequence is operably linked to a nuclear promoter plant that drives expression in a plant cell nucleus； wherein the plastid transgene cassette plant comprising: i) a sequence flanking left and right flanking sequence selected from nucleotide sequences for homologous recombination in plastids； and ii) at least one isolated nucleic acid of interest.Un método para transformar plastidios en una células vegetales, el método comprende: 1) introducir en el núcleo de dicha células vegetales una secuencia de ácidos nucleicos que comprende un promotor nuclear de planta ligado operablemente a una primera secuencia de ácidos nucleicos que comprende un casete de transgén de plastidio, una secuencia de traslocación de plastidios (PTS) es decir una secuencia de ARN capaz de unirse a una proteína de unión de PTS de planta, y un dominio de unión de cebador seleccionado de aquel de un retrotransposón o un retrovirus； 2) introducir en el núcleo de dicha células vegetales una segunda secuencia de ácidos nucleicos que codifica una proteína de unión de secuencia
