METHODS AND COMPOSITIONS TO SUPPORT TISSUE INTEGRATION AND INOSCULATION OF TRANSPLANTED TISSUE AND TRANSPLANTED ENGINEERED PENILE TISSUE WITH ADIPOSE STROMAL CELLS
The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. Another aspect provides a composition comprising an engineered corpus cavernosa tissue comprising SVF cells and corpus cavernosa cells, wherein the SVF cells can be mixed with or encasing the corpus cavernosa cells, and methods of uses thereof, for example in method for the treatment of impotence and erectile dysfunction and/or enhance or contruct a penis. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.La présente invention concerne de manière générale des procédés, des compositions et leurs utilisations destinés à améliorer la vascularisation dun transplant tissulaire ou cellulaire destiné à être transplanté chez un sujet. Un aspect de la présente invention concerne notamment des procédés et des compositions qui comprennent lutilisation dune population de cellules de la fraction vasculaire stromale (SVF) pour encapsuler ou entourer un transplant tissulaire ou cellulaire pour améliorer la vascularisation du transplant tissulaire ou cellulaire. Un autre aspect de la présente invention concerne des procédés et des compositions destinés à améliorer la vascularisation dun transplant tissulaire ou cellulaire par combinaison dune population de cellules de SVF avec un transplant tissulaire ou cellulaire po
