A magneto-optical bio-detection device including: a sample cell, a coil, a magnetic core, a light source and a light detection unit. The sample cell is filled with a solution containing a detection object and a magnetic biosensor capable of combining with the detection object to form a magnetic cluster. The coil is used for producing an oscillating magnetic field. The magnetic core has a guide portion, and an upper magnetic pole and a lower magnetic pole located at both ends of the guide portion; on a cross section orthogonal to the oscillating magnetic field, a cross-sectional area of the upper magnetic pole is less than a cross-sectional area of the guide portion. The light source is used for emitting light rays to penetrate the sample cell. The light detection unit is used for receiving the light rays that penetrated the sample cell to produce a detection signal.
