The invention relates to a method for improving output of paddy rice seed. The method inhibits the expression of LOC_Os08g12430 gene in the DNA of paddy rice genome. The invention also discloses a production method of a paddy rice seed, wherein in the paddy rice seed, the expression of LOC_Os08g12430 gene is inhibit, and a paddy rice seed prepared by the same. According to the method, the output of paddy rice seed is increased by inhibiting the expression of LOC_Os08g12430 gene； no foreign gene is introduced into the paddy rice, the risk caused by foreign is avoided, and the method can be used to produce a large amount of paddy rice seeds, which are safer.
