Disclosed are a centrifugation method and a syringe for centrifugation whereby it is possible to subject a processing target substance to relatively rapid and straightforward centrifugation. A centrifugation method using a syringe having a nozzle formed in one end thereof comprises the steps of: storing a processing target substance inside the syringe carrying out a first centrifugation of the processing target substance which is inside the syringe discharging, via the nozzle, one specific component which has come to be disposed towards the nozzle because of the first centrifugation disposing the same syringe in the other orientation, and carrying out a second centrifugation of the processing target substance which is inside the syringe and discharging, via the nozzle, another specific component which has come to be disposed towards the nozzle because of the second centrifugation.