A novel fundus angiographic application and method using fluorescein isothiocyanate dextrans (FITC-dextran) as fluorescence angiographic dye for diagnosis and evaluation of eye diseases on humans is disclosed. The method involves the process of constitution of FITC-dextran solution and intravenous injection of the said solution, followed by observation and photography of ocular fundus circulation using a fluorescence fundus camera with an image system. The invention offers improved fundus angiograms over the traditional Na-fluorescein fundus angiography (Na-fluorescein) and indocyanine green fundus angiography (ICG) with the advantages of higher clarity and higher resolution capable of visualizing both the retinal and the choroidal circulations systems simultaneously providing longer fundus image duration so that it can be used as a directly mapping guidance in retinal surgery or to complete the angiograms for both eyes by a single process providing more information about the pathological changes occurred among retinal, sub-retinal and choroidal circulation systems.
