The invention relates to the field of disinfectants, in particular to a botanical disinfection composition. The botanical disinfection composition comprises the following raw materials in parts by weight: 0.5-1.0 part of tea tree essential oil, 0.5-1.0 part of niaouli essential oil, 0.5-1.0 part of palmarosa essential oil, 0.5-1.0 part of lemon mint essential oil, and 0.3-0.8 part of lemon myrtle essential oil and/or litsea cubeba essential oil. The invention further provides a method for preparing the botanical disinfection composition, comprising uniformly mixing tea tree essential oil, niaouli essential oil, palmarosa essential oil, lemon mint essential oil, and lemon myrtle essential oil and/or litsea cubeba essential oil according to predetermined parts by weight. The botanical disinfection composition provided by the invention is low in usage amount of ethanol, small in toxic and side effects, good in sterilization effect and short in action time.
