СМИТ Мария Лея (US),САСМАН Джанго (US),АРТУР Уильям (US),НЕСТЕРОВА Альбина (US)
申请号：
RU2013130609/10
公开号：
RU2013130609A
申请日:
2011.12.06
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. Humanitariannet antibody containing a mature variable region of the heavy chain having an amino acid sequence of at least 90% identical to SEQ ID NO: 53, provided that the position H27 is occupied L, position H29 is I, H30 - E and H94 - V and a mature light chain variable region at least 90% identical to SEQ ID NO: 60, provided that position L36 is Y and position L46 is P.2. The humanized antibody of claim 1, comprising three hypervariable CDR regions from SEQ ID NO: 53 and three CDRs from SEQ ID NO: 60.3. The humanized antibody according to claim 1 or 2, with the additional condition that the H76 position is occupied N.4. The humanized antibody of claim 1, comprising a mature variable region of the heavy chain having an amino acid sequence of at least 95% identical to SEQ ID NO: 53, and a mature variable region of the light chain of at least 95% identical to SEQ ID NO: 60.5 . The humanized antibody of claim 1, wherein the mature variable region of the heavy chain is fused to a constant region of the heavy chain, and the mature variable region of the light chain is fused to a constant region of the light chain. The humanized antibody of claim 1, wherein the heavy chain constant region is a mutant form of a natural human constant region that has reduced binding to the Fc-gamma receptor compared to the natural human constant region. The humanized antibody of claim 1, wherein the constant region of the heavy chain is of the IgG1.8 isotype. The humanized antibody according to any one of paragraphs. 1 or 7, where the constant region of the heavy chain has an amino acid sequence containing SEQ ID NO: 44, and the constant region of the light chain has an amino acid sequence, with1. Гуманизированное антитело, содержащее зрелый вариабельный участок тяжелой цепи, имеющий аминокислотную последовательность, по меньшей мере на 90% идентичную SEQ ID NO: 53, при условии, что положение Н27 занято L, положение Н29 занято I, Н30 - Е и Н94 - V, и зрелый вариабельный участок легкой
