Methods of repairing and regenerating hard or soft tissue and reducing inflammation, anti-angiogenesis and anti-osteogenesis in a mammal and related methods of removing PF4 from platelets containing blood preparations. In one embodiment, the method comprises administering to a mammal platelets containing blood preparation where the PF4 has been reduced from the platelet containing blood preparation to repair and regenerate hard and soft tissue in the mammal.
