SCREENING METHOD USING, AS INDEX, EXPRESSION LEVEL OF OCCULUDIN GENE, CLAUDIN GENE AND ZO-1 GENE UNDER HYPOXIC CONDITIONS, AGENT FOR INHIBITING DECREASE IN EXPRESSION LEVEL OF CLAUDIN GENE, AGENT FOR INHIBITING DETERIORATION OF FUNCTIONS OF CELL ADHESION DEVICE, AND AGENT FOR IMPROVING OR PREVENTING DETERIORATION OF SKIN BARRIER FUNCTIONS
PROBLEM TO BE SOLVED: To provide a new technique for screening a component having the effects of improving or preventing the skin aging phenomenon, particularly, deterioration of skin barrier functions caused by aging.SOLUTION: The present invention uses, as indexes, the effects of inhibiting a decrease in expression levels of occuludin gene, claudin gene, zo-1 gene and cadherin gene in cells cultured under hypoxic conditions.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2020,JPO&INPIT【課題】肌の老化現象、特に加齢に伴う皮膚バリア機能の低下を改善又は予防する効果のある成分をスクリーニングするための新たな技術を提供する。【解決手段】低酸素条件で培養した細胞におけるｏｃｃｕｌｕｄｉｎ遺伝子、ｃｌａｕｄｉｎ遺伝子、ｚｏ－１遺伝子及びｃａｄｈｅｒｉｎ遺伝子の発現低下の抑制効果を指標とする。【選択図】図１
