The present invention provides an automatic insect body fluid collecting machine, which comprises a conveying device, a carrier and a positioning cutting system all configured on a machine frame. The conveying device comprises a rail, a chuck base and a chuck, in which the chuck base may move horizontally on the rail, and the chuck may move upward and downward on the chuck base. During operation, the insect holders are placed on the carrier making all the insects in the insect holders having their backs facing the direction of the cutting device. The chuck may grip the insect holder to the cutting device, and sequentially have the back of each insect opposite to the cutting device. Thus, when the cutting device cut an incision on the insect surface communicating with the back artery, it will not cut to the viscera between the back artery and the abdominal region, such that the collected insect body fluid will not be mixed with the viscera fluid.本發明為一種自動昆蟲體液收集機,其係於一機架上設有一輸送裝置、一放置台及一定位切割系統;該輸送裝置包括有一軌道、一夾具座及一夾具,該夾具座可於軌道上進行橫向的位移,該夾具可於夾具座上朝向上方或下方進行位移;使用時於該放置台上放置昆蟲夾持器,昆蟲夾持器內的昆蟲皆以背部朝向於切割裝置的方向,該夾具可將昆蟲夾持器夾持至切割裝置處,並依序將各昆蟲的背部相對於切割裝置,藉此切割裝置將昆蟲表面切出與背部動脈管連通的切口時,不會切割至背部動脈管與腹部間的內臟,使收集得到的昆蟲體液不會混雜有內臟臟液。23‧‧‧夾具231‧‧‧夾臂30‧‧‧放置台301‧‧‧放置槽31‧‧‧昆蟲夾持器313‧‧‧容置槽41‧‧‧攝影裝置60‧‧‧昆蟲