A detection method for detecting blood glucose and whole blood hemoglobin includes the following steps: applying a first voltage to a blood sample and obtaining a first level of blood glucose applying a second voltage to the blood sample and obtaining a second level of blood glucose applying a third voltage to the blood sample and obtaining a hematocrit index and a hemoglobin index of the blood sample transforming the hematocrit index into a hematocrit value which further calibrating the second level of blood glucose and transforming the hemoglobin index into a hemoglobin value. The present invention is advantageous for simultaneously detecting the blood glucose and hemoglobin by single meter with single strip.一種檢測方法,應用於檢測一血液樣本之血糖值及血紅素值,包括以下步驟:對血液樣本施加一第一電壓,取得一第一血糖數值;對血液樣本施加一第二電壓,取得一第二血糖數值;對血液樣本施加一第三電壓,取得血液樣本之一血球容積比指標及一血紅素指標;轉換血球容積比指標為一血球容積比值,並以血球容積比值校正第二血糖數值;以及轉換血紅素指標為一血紅素值。本發明具有可於單一檢測儀上,利用單一試片同時檢測血液樣本之血糖值及血紅素值之優勢。S11~S19‧‧‧步驟