A system for irradiating a microplate may include a light engine with a plurality of light sources, such as light-emitting diodes, included in one or more linear arrays. The plurality of light sources are configured to emit germicidal irradiation, which is directed to the microplate by optical components, such as optical lenses positioned on top of each well of the microplate. The linear array is linearly movable so that as the linear array scans across the microplate, the optical components direct the germicidal irradiation to a plurality of surfaces of each well.