CPG-OLIGODEOXYNUCLEOTIDE, IMMUNOGENIC COMPOSITION COMPRISING THE SAME, AND METHODS FOR PREPARING THE COMPOSITION AND STIMULATING IMMUNE RESPONSE THEREBY
CpG-oligodeoxynucleotides (CpG-ODN), as potent immune stimuli developed for using as adjuvant in different species, are disclosed herein. TLR9 is the cellular receptor for CpG-ODN, and current developed CpG-ODN has low activity to rabbit TLR9. Here, a type of CpG-ODN containing a GACGTT or AACGTT motif in about 11-14 deoxynucleotides was demonstrated to have potent immunostimulatory activity to rabbit TLR9, and capable of boosting a less toxic and potent antibody response in rabbits.於此揭露一種CpG寡去氧核苷酸(CpG-ODN),其係作為開發用於在不同物種中使用作為佐劑的有效免疫刺激。TLR9為CpG-ODN的細胞受體,且當前開發的CpG-ODN對兔子的TLR9具有低活性。本文中,包含約11-14個去氧核苷酸的GACGTT或AACGTT基序之CpG-ODN類型係展現出對兔子的TLR9具有有效之免疫刺激活性,且能夠在兔子中提升較少毒性且有效的抗體反應。
