NAM, DO HYUN,HONG, SEUNG CHYUL,KANG, BONG GU,JOO, KYEUNG MIN
申请号：
CA2788133
公开号：
CA2788133C
申请日:
2011.02.01
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention relates to stem cells in which agene that activates signaling is introduced and to a methodfor proliferating the stem cells. More specifically, theinvention relates to a method of significantly increasing theability of stem cells to proliferate, either by transfectingstem cells with the Notch intracellular domain (NICD) toactivate the Notch signaling pathway, or by transfecting stemcells with the c-MET gene and treating the transfected stemcells with the HGF ligand protein to activate the c-MET/HGFsignaling pathway. According to the present invention, as aresult of activating the Notch signaling pathway or the c-MET/HGFsignaling pathway, stem cells having an excellentability to proliferate can be produced in large amounts.Particularly, since neural stem cells which have beendifficult to culture in vitro can be proliferated in largeamounts, thus the neural stem cells will be more useful forthe preparation of cell therapeutic agents for treatingcranial nerve diseases.
