A composition of Lactobacillus fermentum GMNL-296 and a method for improving the inflection symptoms of Clostridium difficile are provided. The Lactobacillus fermentum GMNL-296 is capable of promoting the expression of anti-inflammatory cytokine IL-10 and Treg cell related transcription factor, so that the inflection symptoms of weight loss and intestinal abnormalities are improved.本發明提供一種改善困難梭狀桿菌感染症狀的發酵乳酸桿菌GMNL-296組合物及方法。該發酵乳酸桿菌GMNL-296可以促進抗發炎細胞激素IL-10及調節型T細胞相關轉錄因子之表達而改善體重減輕及腸道異常的症狀。