Disclosed are a dental barrier membrane for guided bone regeneration using a silk matrix and a method of manufacturing the same, wherein the dental barrier membrane is configured such that a silk matrix having a cross-section with a first thickness, produced from silkworms, is subjected to planar division into two or more silk matrix pieces having a predetermined shape with the first thickness. This dental barrier membrane is biocompatible, has superior tensile strength and porosity, and promotes the formation of new bone in bone defects, unlike existing dental barrier membranes.
