To provide a bone tissue regeneration sheet having a cortical bone tissue layer of 200µm or more in thickness, the bone tissue regeneration sheet is produced by seeding chondroblasts or stem cells for differentiating to chondrocytes on one side of a porous bioabsorbable polymer sheet, taking the seeded porous body into a culture medium, applying centrifugal force of 100 to 1000G to the culture medium for a predetermined time so as to aggregate the chondroblasts or the stem cells for differentiating to chondrocytes, culturing the aggregated cells in a culture medium not containing serum but containing one or two or more kinds selected from ascorbic acid, an ascorbic acid derivative, and dexamethasone without applying centrifugal force to form a chondrocyte layer having a thickness of 200µm or more.
