A specimen cup system includes a cup defining first and second reservoirs in fluid communication with one another. The first reservoir has an open top with spaced-apart upper and lower annular flanges defined at the open tops external periphery. A test card is permanently disposed in the second reservoir. A base, rotatably coupled to the cup, defines a chamber having an opening therein. The opening of the chamber is in fluid communication only with the first reservoir when the base is in a first position, and is in fluid communication only with the second reservoir when the base is in a second position. A seal, rim, and lid cooperate with the upper and lower annular flanges of the open top of the first reservoir to seal the first reservoir after a specimen has been deposited therein.