The present disclosure is directed to a method of preparing an osteogenic bone graft. The method includes introducing an anticoagulant compound into a volume of cellular material including a mononuclear cell population from the intramedullary canal of a long bone collecting an effluent including the volume of cellular material containing the anticoagulant compound at a collection point separating a concentrated cell fraction from the effluent, the concentrated cell fraction including the mononuclear cell population and the anticoagulant compound and preparing an osteogenic bone graft from the concentrated cell fraction.
