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Synthetic gene
专利权人:
DOW AGROSCIENCES LLC
发明人:
申请号:
ARP120101262
公开号:
AR086006A1
申请日:
2012.04.12
申请国别(地区):
AR
年份:
2013
代理人:
摘要:
Synthetic codon optimized sequences being used for plants basically in the same Frequency to which it is used, on average, in reporting genes in natural plant species.The application also includes Synthetic DNA Sequence for herbicide Tolerance, tolerance to water stress and / or due to heat, changes of Healthy Oils and are used for processing the Marker Gene and selectable Marker Genes.Describes the Construction of transgenic plants containing DNA and Synthetic sequences as well as methods and compositions for the use of plants in Agriculture.Claim 1: a sequence of Synthetic DNA to express a Protein of interest in native Maize cells comprising: a) a DNA Sequence encoding the optimized codon - Native Protein of interest, (b) at least one selected from the polyadenylation Signal sequence Group consisting of Class I and class II where; Class I is selected from the group consisting aataaa,Aataat, aaccaa, Atataa, aatcaa, atacta, ATAAAA, atgaaa, aagcat, attaat, atacat, attaaa aattaa AATACA aaaata,,,, and cataaa; and class II selected from the group consisting atatat, ttgttt, ttttgt, tgtttt, Tatata, tatttt, tttttt, attttt, ttattt, tttatt, Taataa, atttat, tatatt, ttttat, atattt, tat Tat, tgtttg, ttatat, tgtaat,And aaataa; and where the native DNA Sequence contains at least a polyadenylation Signal sequence of Class II and where the sequence of Synthetic DNA contains less number of polyadenylation Signal sequences of Class II that the DNA sequence of the native n Protein Ative and contains the same amount of polyadenylation Signal sequences of Class I in comparison withThis sequence of native DNA.Las secuencias sintéticas reivindicadas utilizan codones optimizados para plantas básicamente en la misma frecuencia a la cual se utilizan, en promedio, en genes de presentación natural en la especie de planta. La solicitud incluye además secuencia de ADN sintético para tolerancia a herbicidas, tolerancia al estrés por falta de agua y/o debido al calor, modificaciones de aceites sa
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