The testing for the Lyme disease pathogen, (Borrelia bergdorferi (Bb) and all other Lyme Borrelia), is notoriously difficult. Bb is not by nature a blood loving bacteria, and prefers the climate of avascular tissues such as cartilage. The Borrelia Provocation Procedure disclosed herein proposes to uniquely lure the Lyme Borrelia spirochetes into the blood through a simple procedure of sub-dermal injection of benign tick saliva and co-factors. Lyme Borrelia can then be tested accurately after the provocation injection (BPP), utilizing any Lyme Borrelia detection test ideally as a specific PCR (polymerase chain reaction) test for the most accurate results as there will be abundantly available DNA present in the blood if there is an existing infection. Treatment is more effective after provocation as well, due to the same action of the Bb. The provocation protocol taught herein makes accurate, non-invasive, active direct Lyme infection testing possible, and creates a platform for an effective treatment as well.