LVOV, Vyacheslav Leonidovich;ЛЬВОВ, Вячеслав Леонидович;АПАРИН, Петр Геннадьевич;APARIN;ELKINA, Stanislava Ivanovna;APARIN, Petr Gennadievich;ЕЛКИНА, Станислава Ивановна;GOLOVINA, Marina Eduardovna; Petr Gennadievich;ГОЛОВИНА,
发明人:
APARIN, Petr Gennadievich,АПАРИН, Петр Геннадьевич,LVOV, Vyacheslav Leonidovich,ЛЬВОВ, Вячеслав Леонидович,ELKINA, Stanislava Ivanovna,ЕЛКИНА, Станислава Ивановна,GOLOVINA, Marina Eduardovna,ГОЛОВИНА,
申请号：
RURU2011/000314
公开号：
WO2012/154072A1
申请日:
2011.05.06
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
For the first time, an O-specific polysaccharide antigen that is a Shigella sonnei phase I exopolysaccharide has been produced and characterized, said exopolysaccharide being an authentic natural compound in the form of a bacterial capsular polysaccharide consisting of 1-100 repeating disaccharide units of 0-[4-amino-2-(N-acetyl)amino-2,4- dideoxy-β-D-galactopyranosyl]-(1→4)-0-[2-(N-acetyl)amino-2-deoxy-α-L-altrpyranuronic acid] linked by (1→3) bonds to form a polysaccharide chain. The exopolysaccharide has a molecular mass of from 0.4 to 400 kDa, contains a non-toxic lipid component, namely non-hydroxylated fatty acids, and exhibits low pyrogenicity and high immunogenicity, generating mucosal protection against Shigella sonnei shigellosis by inducing the synthesis of specific antibodies against S. sonnei in mammals, including humans. Without using lipopolysaccharides as the source of production, an exopolysaccharide with a high degree of purity is produced from a liquid phase culture of S. sonnei bacteria by means of a workable industrial method with a high yield. Effective, highly specific and safe vaccines for the prophylaxis and/or treatment of Shigella sonnei shigellosis are developed on the basis of the above-mentioned polysaccharide, as well as pharmaceutical compositions with a broad spectrum of action, in particular, in modulating immune response.Pour la première fois, un antigène de polysaccharide O spécifique qui est un exopolysaccharide en phase I de Shigella sonnei a été produit et caractérisé comme étant un composé naturel authentique se présentant sous la forme d'un polysaccharide capsulaire bactérien constitué de 1 à 100 unités de répétition disaccharide de l'acide 0-[4-amino-2-(N-acetyl)amino-2,4- didésoxy-β-D-galactopyranosyl]-(1→4)-0-[2-(N-acetyl)amino-2-deoxy-α-L-altrpyranuronique] lié par (1 à 3) liaisons de façon à former une chaîne polysaccharide. L'exopolysaccharide a une masse moléculaire comprise entre 0,4 et 400 kDa, contient un composant
