Deutsches Primatenzentrum GmbH (DPZ) - Leibniz-Institut für Primatenforschung
发明人:
申请号：
EP18704478.9
公开号：
EP3577217A1
申请日:
2018.02.01
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
The present invention relates to a method of producing a polyomavirus or polyomavirus-derived virus-like particle (VLP) carrying on its surface at least one targeting molecule that binds to a cell of interest, the method comprising the step of contacting the polyomavirus or polyomavirus-derived VLP with (i) the targeting molecule, wherein the at least one targeting molecule is glycosylated with at least one glycosyl residue that is recognised by the polyomavirus or polyomavirus-derived VLP； or (ii) a first interaction molecule, wherein the first interaction molecule is glycosylated with at least one glycosyl residue that is recognised by the polyomavirus or polyomavirus-derived VLP； and the at least one targeting molecule, wherein the at least one targeting molecule is conjugated to a second interaction molecule capable of interacting with the first interaction molecule. The present invention further relates to a polyomavirus or polyomavirus-derived virus-like particle (VLP), wherein the virus or VLP carries on its surface at least one targeting molecule that binds to a cell of interest, as well as to a polyomavirus or polyomavirus-derived VLP obtained or obtainable by the method of the invention. Furthermore, the present invention relates to a composition comprising said polyomavirus or polyomavirus-derived VLP and to the use of the polyomavirus or polyomavirus-derived VLP of the invention or the composition of the invention for use as a medicament. The present invention further relates to a kit comprising the polyomavirus or polyomavirus-derived VLP or the composition of the invention.La présente invention concerne une méthode de production d'un polyomavirus ou d'une particule pseudo-virale (PPV) dérivée du polyomavirus portant sur sa surface au moins une molécule de ciblage qui se lie à une cellule d'intérêt, la méthode comprenant l'étape consistant à mettre en contact le polyomavirus ou la PPV dérivée du polyomavirus avec (i) la molécule de ciblage, la ou lesdit
